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The 2nd response cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circularly polarized luminescence. As attracted Fig - https://papaly.com/categories/share?id=82b22f606fb5496cbfab4f86fdfbca1c. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As revealed in Fig. 9, this might be since the H4B radical serve as an electron sink in a later point in the response, so as to permit NOS to, Fig. 9. published here A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.